ABSTRACT
A simple and rapid spectrophotometric assay for the determination of metoclopramide hydrochloride in pure and dosage forms has been developed. It is based on the reaction between metoclopramide and 3-methyl-2-benzothiazolone hydrazone in the presence of ferric chloride to give a coloured product with a maximum absorbance at 556 nm. The results obtained are reproducible with a coefficient of variation less than 2% and in a good agreement with the official method. The proposed method is also applicable to urine samples
Subject(s)
Spectrophotometry/methodsABSTRACT
A simple, rapid and accurate first-derivative spectrophotometric method was described for the simultaneous determination of sulfamethoxazole and its metabolite N4-acetylsulfamethoxazole in urine. The metabolite N4-acetylsulfamethoxazole was determined by measuring the trough amplitude at gamma 242 nm, while the free acid [sulfamethoxazole] was determined from the peak amplitude at gamma 272 nm after correction. Analysis of mixtures containing variable concentrations of both compounds gave recovery percentages of 96.4 and 96.3, respectively. The described method was successfully utilized for the determination of the cumulative amounts of sulfamethoxazole and its metabolite in urine following an oral dose of 400 mg sulfamethoxazole to a human male volunteer
Subject(s)
Urine , Spectrophotometry/methodsABSTRACT
Derivative spectrophotometric methods were described for the determination of metoclopramide HCl and lignocaine HCl in dosage forms. The first drug was determined in suppositories, syrups, ampoules, tablets and drops. The second was determined in vials, jelly and ointments. The methods were efficient to correct for the interference due to additives viz. parabens and coadministered drugs as paracetamol and naphazoline. Results obtained were accurate with coefficient of variation less than 2%